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KMID : 0371619950110020247
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Journal of Wonkwang Medical Science
1995 Volume.11 No. 2 p.247 ~ p.252
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The Role of Cumulus Cell in Spontaneous maturation In Vitro of oocyte and In Vitro Fertilization of the Mouse
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Rhee Hee-Sub
Hong Kee-Yeon
Lee Hee-Min
Choi Eun-Ha
Kim Won-Sin
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Abstract
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This study was to determine whether cumulus cell augments spontaneous maturation in vitro of oocyte and in vitro fertilization of the mouse. The maturation rate was evaluated by analyzing the metaphase Ⅱ chromosomal configurations 24 hour after incubation and the fertilization frequency was evaluated by observation of zygotic division to 2-cell 16 hour after insemination. The maturation rate of cumulus-oocyte complex was not significantly different in that of denuded oocytes(86% versus 81%). The fertilization frequency of cumulus-oocyte complex was significantly higher than that of denuded oocytes (59% versus 29%, p<0.05). It is concluded that the presence of cumulus cell does not augment the spontaneous maturation of immature oocyte but augments the fertilization frequency in mouse.
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